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Effect of PTP on oxidative stress in RAW 264.7 macrophages. To measure ROS and <t>SOD</t> concentration, the cells were pretreated with PTP (25, 50, and 100 μ g/mL), DEX (10 μ g/mL), or IBU (10 μ g/mL), then treated with LPS (0.1 μ g/mL). ROS production was detected with a fluorescence microplate reader (Ex. 485 nm; Em. 535 nm) (a), (b). To measure MDA contents, 200 μ L of thiobarbituric acid (TBA) was added to each sample, after which absorbance was measured at 532 nm (c). SOD concentration was measured with an <t>ELISA</t> kit (d). SOD1 and SOD2 production were measured by western blot (e), (f). SOD1 and SOD2 relative density was analyzed using densitometry. All values were reported as the mean ± SD. # indicates a significant difference from the negative control group (# P < 0.05, ## P < 0.01, ### P < 0.001). ∗ indicates a significant difference from the positive control group ( ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). PTP: Pinus thunbergii Parl.; DEX: dexamethasone; IBU: ibuprofen; CA: caffeic acid.
Elisa Kits Sod, supplied by Cloud-Clone corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Abbkine Inc rat sod elisa kits
Effect of PTP on oxidative stress in RAW 264.7 macrophages. To measure ROS and <t>SOD</t> concentration, the cells were pretreated with PTP (25, 50, and 100 μ g/mL), DEX (10 μ g/mL), or IBU (10 μ g/mL), then treated with LPS (0.1 μ g/mL). ROS production was detected with a fluorescence microplate reader (Ex. 485 nm; Em. 535 nm) (a), (b). To measure MDA contents, 200 μ L of thiobarbituric acid (TBA) was added to each sample, after which absorbance was measured at 532 nm (c). SOD concentration was measured with an <t>ELISA</t> kit (d). SOD1 and SOD2 production were measured by western blot (e), (f). SOD1 and SOD2 relative density was analyzed using densitometry. All values were reported as the mean ± SD. # indicates a significant difference from the negative control group (# P < 0.05, ## P < 0.01, ### P < 0.001). ∗ indicates a significant difference from the positive control group ( ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). PTP: Pinus thunbergii Parl.; DEX: dexamethasone; IBU: ibuprofen; CA: caffeic acid.
Rat Sod Elisa Kits, supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MultiSciences Biotech Co Ltd elisa kits for superoxide dismutase (sod)
Effect of PTP on oxidative stress in RAW 264.7 macrophages. To measure ROS and <t>SOD</t> concentration, the cells were pretreated with PTP (25, 50, and 100 μ g/mL), DEX (10 μ g/mL), or IBU (10 μ g/mL), then treated with LPS (0.1 μ g/mL). ROS production was detected with a fluorescence microplate reader (Ex. 485 nm; Em. 535 nm) (a), (b). To measure MDA contents, 200 μ L of thiobarbituric acid (TBA) was added to each sample, after which absorbance was measured at 532 nm (c). SOD concentration was measured with an <t>ELISA</t> kit (d). SOD1 and SOD2 production were measured by western blot (e), (f). SOD1 and SOD2 relative density was analyzed using densitometry. All values were reported as the mean ± SD. # indicates a significant difference from the negative control group (# P < 0.05, ## P < 0.01, ### P < 0.001). ∗ indicates a significant difference from the positive control group ( ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). PTP: Pinus thunbergii Parl.; DEX: dexamethasone; IBU: ibuprofen; CA: caffeic acid.
Elisa Kits For Superoxide Dismutase (Sod), supplied by MultiSciences Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ZellBio GmbH elisa kits sod
Effect of PTP on oxidative stress in RAW 264.7 macrophages. To measure ROS and <t>SOD</t> concentration, the cells were pretreated with PTP (25, 50, and 100 μ g/mL), DEX (10 μ g/mL), or IBU (10 μ g/mL), then treated with LPS (0.1 μ g/mL). ROS production was detected with a fluorescence microplate reader (Ex. 485 nm; Em. 535 nm) (a), (b). To measure MDA contents, 200 μ L of thiobarbituric acid (TBA) was added to each sample, after which absorbance was measured at 532 nm (c). SOD concentration was measured with an <t>ELISA</t> kit (d). SOD1 and SOD2 production were measured by western blot (e), (f). SOD1 and SOD2 relative density was analyzed using densitometry. All values were reported as the mean ± SD. # indicates a significant difference from the negative control group (# P < 0.05, ## P < 0.01, ### P < 0.001). ∗ indicates a significant difference from the positive control group ( ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). PTP: Pinus thunbergii Parl.; DEX: dexamethasone; IBU: ibuprofen; CA: caffeic acid.
Elisa Kits Sod, supplied by ZellBio GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ELK Biotechnology enzyme-linked immunosorbent assay (elisa) kits for sod (bc009)
Effect of PTP on oxidative stress in RAW 264.7 macrophages. To measure ROS and <t>SOD</t> concentration, the cells were pretreated with PTP (25, 50, and 100 μ g/mL), DEX (10 μ g/mL), or IBU (10 μ g/mL), then treated with LPS (0.1 μ g/mL). ROS production was detected with a fluorescence microplate reader (Ex. 485 nm; Em. 535 nm) (a), (b). To measure MDA contents, 200 μ L of thiobarbituric acid (TBA) was added to each sample, after which absorbance was measured at 532 nm (c). SOD concentration was measured with an <t>ELISA</t> kit (d). SOD1 and SOD2 production were measured by western blot (e), (f). SOD1 and SOD2 relative density was analyzed using densitometry. All values were reported as the mean ± SD. # indicates a significant difference from the negative control group (# P < 0.05, ## P < 0.01, ### P < 0.001). ∗ indicates a significant difference from the positive control group ( ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). PTP: Pinus thunbergii Parl.; DEX: dexamethasone; IBU: ibuprofen; CA: caffeic acid.
Enzyme Linked Immunosorbent Assay (Elisa) Kits For Sod (Bc009), supplied by ELK Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effect of PTP on oxidative stress in RAW 264.7 macrophages. To measure ROS and SOD concentration, the cells were pretreated with PTP (25, 50, and 100 μ g/mL), DEX (10 μ g/mL), or IBU (10 μ g/mL), then treated with LPS (0.1 μ g/mL). ROS production was detected with a fluorescence microplate reader (Ex. 485 nm; Em. 535 nm) (a), (b). To measure MDA contents, 200 μ L of thiobarbituric acid (TBA) was added to each sample, after which absorbance was measured at 532 nm (c). SOD concentration was measured with an ELISA kit (d). SOD1 and SOD2 production were measured by western blot (e), (f). SOD1 and SOD2 relative density was analyzed using densitometry. All values were reported as the mean ± SD. # indicates a significant difference from the negative control group (# P < 0.05, ## P < 0.01, ### P < 0.001). ∗ indicates a significant difference from the positive control group ( ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). PTP: Pinus thunbergii Parl.; DEX: dexamethasone; IBU: ibuprofen; CA: caffeic acid.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Pinus thunbergii Parl. Extracts Reduce Acute Inflammation by Targeting Oxidative Stress

doi: 10.1155/2021/7924645

Figure Lengend Snippet: Effect of PTP on oxidative stress in RAW 264.7 macrophages. To measure ROS and SOD concentration, the cells were pretreated with PTP (25, 50, and 100 μ g/mL), DEX (10 μ g/mL), or IBU (10 μ g/mL), then treated with LPS (0.1 μ g/mL). ROS production was detected with a fluorescence microplate reader (Ex. 485 nm; Em. 535 nm) (a), (b). To measure MDA contents, 200 μ L of thiobarbituric acid (TBA) was added to each sample, after which absorbance was measured at 532 nm (c). SOD concentration was measured with an ELISA kit (d). SOD1 and SOD2 production were measured by western blot (e), (f). SOD1 and SOD2 relative density was analyzed using densitometry. All values were reported as the mean ± SD. # indicates a significant difference from the negative control group (# P < 0.05, ## P < 0.01, ### P < 0.001). ∗ indicates a significant difference from the positive control group ( ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). PTP: Pinus thunbergii Parl.; DEX: dexamethasone; IBU: ibuprofen; CA: caffeic acid.

Article Snippet: ELISA kits for SOD (Cloud‐Clone Corp., Katy, TX, USA) were used according to the manufacturer's protocol.

Techniques: Concentration Assay, Fluorescence, Enzyme-linked Immunosorbent Assay, Western Blot, Negative Control, Positive Control